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A novel mutation that affects utilization of galactose in Saccharomyces cerevisiae

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Galactose metabolism in Saccharomyces cerevisiae is regulated by a complex interplay of genetic factors, and understanding these mechanisms is crucial for applications in biotechnology and fermentation processes. This study identifies a novel recessive mutation, named gal11, which significantly reduces the activity of key galactose-utilizing enzymes, rendering mutant cells unable to ferment galactose, thereby providing insights into the regulatory pathways governing galactose metabolism.

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Summary

A novel type of regulatory mutation for galactose metabolism in Saccharomyces cerevisiae is described. The mutation named gal11 was recessive, non-allelic to GAL4, GAL80, GAL2, or GAL3, and unlinked to the gene cluster of GAL1, GAL10, and GAL7. It caused a ‘coordinate’ reduction of galactokinase, galactose-1-P uridylyl transferase, and UDP-glucose 4-epimerase by a factor of more than 5, rendering the mutant cells galactose-nonfermenting. The effect of the mutation was manifested not only in cells grown on galactose but also in cells constitutively synthesizing the galactose-metabolizing enzymes.

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Authors and Affiliations

  1. Research Unit for Molecular Genetics, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan

    Yasuhisa Nogi & Toshio Fukasawa

Authors
  1. Yasuhisa Nogi
  2. Toshio Fukasawa

Additional information

This work was supported in part by a grant (Project No. 410712) from the Ministry of Education, Culture, and Science or Japan

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Nogi, Y., Fukasawa, T. A novel mutation that affects utilization of galactose in Saccharomyces cerevisiae . Curr Genet 2, 115–120 (1980). https://doi.org/10.1007/BF00420623

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  • DOI: https://doi.org/10.1007/BF00420623

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