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⇱ GABAergic inhibition of crayfish deep extensor abdominal muscle exhibits a steep dose-response relationship and a high degree of cooperativity | Pflügers Archiv - European Journal of Physiology | Springer Nature Link

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GABAergic inhibition of crayfish deep extensor abdominal muscle exhibits a steep dose-response relationship and a high degree of cooperativity

  • Original Article
  • Neurophysiology, Muscle and Sensory Organs
  • Published:

Abstract

A patch-clamp study was done to characterize the recently found GABAergic (i.e. γ-aminobutyric acid) inhibitory synaptic channels of crayfish deep extensor abdominal muscle. Outside-out patches were rapidly activated by GABA to measure the dose/ response curves for the open probability of the channels, P o, and the rise time, t r, (time from P o=0.1 to P o=0.9). In some of the patches the GABA-activated currents decayed due to desensitization and such patches were not studied further. Rare channel openings were elicited with 0.1 mM GABA. The P o at this low concentration of GABA was 0.0005 to 0.01. Application of 10 mM GABA was necessary to reach the maximal P o of 0.9. The slope of the dose/response relationship in the double logarithmic plot was 5.4±1.1 (mean±SD; n=9) between 0.1 mM and 0.2 mM GABA. The plot of t r versus GABA concentration had a peculiar shape, recently found to be characteristic for positive cooperativity of the binding sites. t r increased from a minimum at 10 mM GABA with declining concentrations of GABA and reached a peak at 0.4 mM GABA. Below 0.4 mM GABA, t r decreased again. With 0.2 mM GABA t r was 0.40±0.1 (mean±SD; n=4) of the peak value measured at 0.4 mM GABA. Simulations were compared with the experimental results and a linear reaction scheme with five binding sites for GABA was established to describe the dose/response curves for P o and t r.

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Authors and Affiliations

  1. Physiologisches Institut der Technischen Universität München, Biedersteinerstrasse 29, D-80802, Munich, Germany

    Nicolas von Beckerath, Helmuth Adelsberger, Franz Parzefall & Josef Dudel

  2. Neurologische Klinik der Technischen Universität München, Möhlstrasse 28, D-81675, Munich, Germany

    Christian Franke

Authors
  1. Nicolas von Beckerath
  2. Helmuth Adelsberger
  3. Franz Parzefall
  4. Christian Franke
  5. Josef Dudel

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von Beckerath, N., Adelsberger, H., Parzefall, F. et al. GABAergic inhibition of crayfish deep extensor abdominal muscle exhibits a steep dose-response relationship and a high degree of cooperativity. Pflügers Arch. 429, 781–788 (1995). https://doi.org/10.1007/BF00374801

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  • DOI: https://doi.org/10.1007/BF00374801

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