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⇱ Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491 | Nature

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Abstract

Neisseria meningitidis causes bacterial meningitis and is therefore responsible for considerable morbidity and mortality in both the developed and the developing world. Meningococci are opportunistic pathogens that colonize the nasopharynges and oropharynges of asymptomatic carriers. For reasons that are still mostly unknown, they occasionally gain access to the blood, and subsequently to the cerebrospinal fluid, to cause septicaemia and meningitis. N. meningitidis strains are divided into a number of serogroups on the basis of the immunochemistry of their capsular polysaccharides; serogroup A strains are responsible for major epidemics and pandemics of meningococcal disease, and therefore most of the morbidity and mortality associated with this disease. Here we have determined the complete genome sequence of a serogroup A strain of Neisseria meningitidis, Z2491 (ref. 1). The sequence is 2,184,406 base pairs in length, with an overall G+C content of 51.8%, and contains 2,121 predicted coding sequences. The most notable feature of the genome is the presence of many hundreds of repetitive elements, ranging from short repeats, positioned either singly or in large multiple arrays, to insertion sequences and gene duplications of one kilobase or more. Many of these repeats appear to be involved in genome fluidity and antigenic variation in this important human pathogen.

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Figure 1: Circular representation of the N. meningitidis Z2471 genome.
Figure 2: Types of N. meningitidis repeat.
Figure 3: Structure of selected repeat regions.
Figure 4: Graph showing the relationship between repeat array length and flanking gene function.

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Acknowledgements

This research was funded by The Wellcome Trust.

Author information

Authors and Affiliations

  1. The Sanger Centre, The Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK

    J. Parkhill, K. D. James, S. D. Bentley, C. Churcher, D. Basham, D. Brown, T. Chillingworth, R. M. Davies, P. Davis, K. Devlin, T. Feltwell, N. Hamlin, S. Holroyd, K. Jagels, S. Leather, S. Moule, K. Mungall, M. A. Quail, M.-A. Rajandream, K. M. Rutherford, M. Simmonds, J. Skelton, S. Whitehead & B. G. Barrell

  2. Max-Planck Institut für moleculare Genetik, Ihnestraße 73, Berlin, D-14195, Germany

    M. Achtman, S. R. Klee & G. Morelli

  3. Department of Zoology, Wellcome Trust Centre for the Epidemiology of Infectious Disease, University of Oxford, South Parks Road, Oxford, OX1 3FY, UK

    B. G. Spratt

Authors
  1. J. Parkhill
  2. M. Achtman
  3. K. D. James
  4. S. D. Bentley
  5. C. Churcher
  6. S. R. Klee
  7. G. Morelli
  8. D. Basham
  9. D. Brown
  10. T. Chillingworth
  11. R. M. Davies
  12. P. Davis
  13. K. Devlin
  14. T. Feltwell
  15. N. Hamlin
  16. S. Holroyd
  17. K. Jagels
  18. S. Leather
  19. S. Moule
  20. K. Mungall
  21. M. A. Quail
  22. M.-A. Rajandream
  23. K. M. Rutherford
  24. M. Simmonds
  25. J. Skelton
  26. S. Whitehead
  27. B. G. Spratt
  28. B. G. Barrell

Corresponding author

Correspondence to J. Parkhill.

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Parkhill, J., Achtman, M., James, K. et al. Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Nature 404, 502–506 (2000). https://doi.org/10.1038/35006655

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  • DOI: https://doi.org/10.1038/35006655

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