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⇱ Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea | Acta Pharmacologica Sinica

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Abstract

Aim:

To study whether epigallocatechin gallate (EGCG), a green tea-derived polyphenol, exerted anti-influenza A virus activity in vitro and in vivo.

Methods:

Madin-Darby canine kidney (MDCK) cells were tested. The antiviral activity of EGCG in the cells was determined using hemagglutination assay and qPCR. Time of addition assay was performed to determine the kinetics of inhibition of influenza A by EGCG. The level of reactive oxygen species (ROS) were determined with confocal microscopy and flow cytometry. BALB/c mice were treated with EGCG (10, 20 or 40 mg·kg−1·d−1, po) for 5 d. On the 3rd d of the treatment, the mice were infected with influenza A virus. Histopathological changes, lung index and virus titers in the lungs were determined.

Results:

Treatment of influenza A-infected MDCK cells with EGCG (1.25–100 nmol/L) inhibited influenza A replication in a concentration-dependent manner (the ED50 value was 8.71±1.11 nmol/L). Treatment with EGCG (20 nmol/L) significantly suppressed the increased ROS level in MDCK cells following influenza A infection. In BALB/c mice infected with influenza virus, oral administration of EGCG (40 mg·kg−1·d−1) dramatically improved the survival rate, decreased the mean virus yields and mitigated viral pneumonia in the lungs, which was equivalent to oral administration of oseltamivir (40 mg·kg−1·d−1), a positive control drug.

Conclusion:

The results provide a molecular basis for development of EGCG as a novel and safe chemopreventive agent for influenza A infection.

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Acknowledgements

This work was supported by the National Mega Project on Major Drug Development (2009ZX09301-014-1), and National Natural Science Foundation of China (NSFC Project N 30873104).

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Author notes

  1. Jia-xin Ling and Fei Wei: These authors contributed equally to this paper.

Authors and Affiliations

  1. State Key Laboratory of Virology/Institute of Medical Virology/Research Center of Food and Drug Evaluation/State Laboratory of Antiviral and Tumor of Traditional Chinese Medicine, School of Medicine, Wuhan University, Wuhan, 430071, China

    Jia-xin Ling, Fei Wei, Ning Li, Jin-lin Li, Liang-jun Chen, Yuan-yuan Liu, Fan Luo, Hai-rong Xiong, Wei Hou & Zhan-qiu Yang

Corresponding author

Correspondence to Zhan-qiu Yang.

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Ling, Jx., Wei, F., Li, N. et al. Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea. Acta Pharmacol Sin 33, 1533–1541 (2012). https://doi.org/10.1038/aps.2012.80

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  • DOI: https://doi.org/10.1038/aps.2012.80

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