Jeffreys is a surname that may refer to the following notable people:

• Sir Alec Jeffreys (born 1950), British biologist and discoverer of DNA fingerprinting
• Anne Jeffreys (1923–2017), American actress and singer
• Arthur Frederick Jeffreys (1848–1906), British politician
• Bertha Jeffreys (1903–1999), British physicist a.k.a. Bertha Swirles
• Charles Jeffreys (1877 – unknown), South African shooter
• Edmund Jeffreys (1846–1925), British Royal Navy admiral
• Elizabeth Jeffreys (1941–2023), British Byzantinist
• Ellen Penelope Jeffreys (1827–1904), New Zealand artist
• Garland Jeffreys (born 1943), American musician
• George Jeffreys (composer) (c.1610–1685), composer and organist to Charles I
• George Jeffreys, 1st Baron Jeffreys (1645–1689), British jurist & politician
• George Jeffreys, 1st Baron Jeffreys (British Army officer) (1878–1960), British soldier & politician
• George Jeffreys (pastor) (1889–1962), British religious leader
• Gina Jeffreys (born 1968), Australian singer
• Harold Jeffreys (1891–1989), British mathematician, statistician, geophysicist, and astronomer
• J. G. Jeffreys (1893–1977), Australian-born educator
• John Gwyn Jeffreys (1809–1885), British malacologist
• John R. F. Jeffreys (1918–1944), British mathematician
• Julius Jeffreys (1800–1877), British physician
• Justina Jeffreys (1787–1869), Jamaican-born British gentlewoman
• Paul Jeffreys (1952–1988), English rock musician and bassist
• Rhodri Jeffreys-Jones (born 1942), British historian
• Robin Jeffreys (1890–1963), British Olympic fencer
• Sheila Jeffreys (born 1948), British-born political scientist, academic, & activist
• Stephen Jeffreys (1950–2018), British playwright

• Baron Jeffreys, a title in the English and UK peerages
• Jeffreys prior
• Jefferies (disambiguation)
• Jeffrey (disambiguation)
• Jefferys