Twinkle may refer to:

• Twinkling, the variation of brightness of distant objects

• Twinkle (singer) (1948–2015), born Lynn Annette Ripley, English singer-songwriter
• Twinkle Bajpai, Indian singer, television and film actress
• Twinkle Borge (born 1971), Hawaiian activist
• Twinkle Andress Cavanaugh, president of the Alabama Public Service Commission since 2013
• Twinkle Khanna (born 1973), Indian author and actress

• Twinkle (software), Qt-based VoIP soft phone for Linux
• TWINKLE, a hypothetical integer factorization device
• Twinkle (protein), a protein coded by a gene also called TWNK, C10orf2 or PEO1

• Twinkle (comic), a British comic book, published 1968–1999
• Twinkle (book), by Katharine Holabird
• Twinkle, a character in the video game Puzzle Bobble 3 (a.k.a. Bust-a-Move 99)
• Twinkle, a fictional character in Higglytown Heroes
• Twinkle, one of the cousins from We Love Katamari and Me & My Katamari
• Twinkle, a character in Diana Wynne Jones's novel House of Many Ways
• Twinkle, a recurring fictional character in the British sitcom dinnerladies
• Twinkle, a sword carried by R. A. Salvatore's character Drizzt Do'Urden
• Twinkles, a cartoon elephant appearing in episodes of King Leonardo and His Short Subjects
• Cure Twinkle, a character in Go! Princess PreCure

• Twinkle (EP), EP by Girls' Generation subunit TaeTiSeo, and the title song
• "Twinkle", a pop song by Kumi Koda and Show Luo
• "Twinkle", a 1981 single by Earl Klugh
• "Twinkle", a 1996 single by Whipping Boy (Irish band)
• "Twinkle", a song by Purple Kiss on Hide & Seek
• "Twinkle Song", a song on Miley Cyrus & Her Dead Petz
• Twinkle, Twinkle, Little Star, popular English lullaby

• Twinkles, an Occupy movement hand signal
• Twinkl, a specialist digital education publisher

• Twinkle Stars, a manga
• Twinkle Star Sprites, a video game
• Twinkleshine, a My Little Pony
• Tinkle, an indian magazine for kids