The amplitude of the electroretinogram (ERG) is significantly reduced compared to wild type in adults expressing Cib2^GD15199 under the control of Scer\GAL4^GMR.long (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency). The mutant flies fail to reliably follow fast trains of light pulses at a frequency of approximately 40Hz, whereas wild-type controls only show this failure by approximately 70Hz. The flies fail to sustain a persistent ERG response during prolonged stimulation at low frequency (1.7Hz), in contrast to wild type.

Adults expressing Cib2^GD15199 under the control of Scer\GAL4^GMR.long (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) show no obvious defects in eye morphology when raised under 12 hour light: 12 hour dark conditions. However, if they are raised under constant light conditions for 5 days, they show significant photoreceptor degeneration.

Adults expressing Cib2^GD15199 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or early pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 30-40% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.