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URL: https://flybase.org/reports/FBcl0789007.html

⇱ FlyBase Clone Report: Dmel\CH322-78P14

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FB2026_01 , released March 12, 2026
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General Information
Symbol
Dmel\CH322-78P14
Species
D. melanogaster
Name
CH322-78P14
FlyBase ID
FBcl0789007
Feature type
Computed gene(s)
Collection Status
N/A
Known Problems
none
FlyBase assessment
Library
CHORI-322_BAC
  • Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 21kb inserts.
Strain
Vector
Tissue Source
Stage
Tissue/Position (including subcellular localization)
Reference
Sequence Data of the Insert
Full Length Sequence
Genomic Location
3R:27,190,096..27,212,786 [+]
Total bases
22,691
NCBI
Full Length sequence dataSequence Downloader
Left End Sequence
Total bases
612
NCBI
Left End sequence data
GATCAATAAATGTCAGCTCAAATGAGAGAGATCGTCTGCGGCTTTTCGGATTTTTTAGCCTTTTGGCATCCCAACTTGGC
CGAGTAACTGGGTTAATTTCAACTGACATGGGACCATGTGAACTGAGGCGATATGATCGCGGATTCGCTTGACCAGTTGA
CTGGCTGGATGGCTGCCTTCCTTCCCTGGTTTTCCCTGGAAGTTGGCCATAAATCGTCGCTACAAAATATTTAACTTTTA
TTTACTTCATTAACAACTAAATAGTTGCAGTGGGACCAAGGTGGATCTTTGCTGCCGGCGACTTTTATCGCCTCGCTTAC
CCTAAATCAATGCAATCCCCTCTACCAAGTAGATAAATGTGTGTAATCAATTTGATTGCCCCACGGTCGGACACATCCCG
GTATGCTCTTCTGGGGACTGGGACTCGAACTCGCATGCTCCTCGGATTCTAATGTCTGGCTCTCGAGATCTCTGGCGTAT
TAATCAAATTGTTGTTAAGACCCCTCGCCAGCCCCGCTCCTTCAAACTACCTATAACTTTCCGGCCAACGATTAATTTTT
ATGTATTTCTGAACCGTTGGCTGCCGTTGGCTCTCCGACTCTTTGCCTTTTT
Right End Sequence
Total bases
551
NCBI
Right End sequence data
GATCTGCGACATCGGTGACATCGACAGCGATTTCTTATTGATGTTTATTACAGATCCAAAAAATTGTCGCTTGTTTCGGA
TTTTTATTGTCTGCCGGCTGCGCTGACAAGTGCTACATGTTTTTTCGCTCGTTTCGAATTGATTTTTGGGTTTTTGATTT
GAATTTTGGGTTTTTAGATTTCTCTAAATAATACATAATTAATGTGATTGGAAATTATGGCAACTGTTTTCACAATATCG
TGTAAAGCCAAAGGAGTGTGTGTATACTTATGTAGTGACTGTGCTGTGTGAGTGGCGGAAACTTTTGGCTTAACCCTAAT
AACCTTGTTGCTTTTTAGTGGGACAGCAACAGCAGTTGCTAGTTAAGGCAAGACAAATTGACAAATAACATCACCTCACT
TAACCCGGCTTGCAACGCCTTTTTGTTTGGCCAAAATACCAGTGGCAGTCGACCCGGCCTAAAACGCGCTGTGTGGTAGG
CCACAAAAAGCAGGAAAACGCTCCTCCGAGGTGGAGCTTTAATTAGATGCCCCATGGGAGTATCTTTTTTC
Library Information (1)
Library: CHORI-322_BAC
Description

A BAC library containing genomic DNA fragments (average size of 21 kb) in the attB-P[acman]-CmR-BW vector for efficient transgenesis.

Sample preparation
Protocol

DNA isolated from adult fly tissue was prepared in agarose blocks and partially digested with MboI. 20-25 kb fragments were size-selected by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, and ligated into the BamHi site of the attB-P[acman]-CmR-BW vector, a special adaptation of the P[acman] vector. Libraries were transformed into the E.coli strain "EPI300-T1R".

Comment

A total of 73,728 clones were generated.

Reported As
Symbol Synonyms
CHORI-322_BAC
References (3)
Research paper (2)
Venken et al., 2010, Genetics 186(4): 1111--1125
A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. [FBrf0212670]
Venken et al., 2009, Nat. Methods 6(6): 431--434
Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. [FBrf0209231]
Personal communication to FlyBase (1)
GenetiVision Corporation, 2016-, A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome.
A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome. [FBrf0232631]
External Crossreferences and Linkouts ( 1 )
Linkouts
PACMANFLY - The source for information and resources of tools for the Drosophila community using the P[acman] recombineering technique.
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
CH322-78P14
Secondary FlyBase IDs
References (1)