Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody
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- Volume 97, pages 123–130 (1987)
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Summary
Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immuno-fluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.
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Barthold SW, Smith AL (1984) Mouse hepatitis virus strain-related patterns of tissue tropism in suckling mice. Arch Virol 81: 103–112
Buchmeier MJ, Lewicki HA, Talbot PJ, Knobler RL (1984) Murine hepatitis virus-4 (Strain JHM)-induced neurologic disease is modulatedin vivo by monoclonal antibody. Virology 132: 261–270
Cainelli-Gebara VC (1986) Production et caractérisation d'anticorps monoclonaux contre le virus de l'hépatite murine type 3. MSc Dissertation, Université du Québec, Institut Armand-Frappier, Laval-des-Rapides, Québec, Canada
Fleming SO, Stohlman SA, Harmon RC, Lai MMC, Frelinger JA, Weiner LP (1983) Antigenic relationships of murine coronaviruses: analysis using monoclonal antibodies to JHM (MHV-4) virus. Virology 131: 296–307
Lamontagne L, Dupuy J-M (1984) Persistent infection with mouse hepatitis virus type 3 in mouse lymphoid cell line. Infect Immun 44: 716–723.
Lefrancois L (1984) Protection against lethal viral infection by neutralizing and non-neutralizing monoclonal antibodies: distinct mechanisms of actionin vivo. J Virol 51: 208–214
Levy-Leblond E, Oth D, Dupuy J-M (1979) Genetic study of mouse sensitivity to mouse hepatitis type 3 infection: influence of the H-2 complex. J Immunol 122: 1359–1362
Lussier G, Descôteaux J-P (1986) Prevalence of natural virus infections in laboratory mice and rats used in Canada. Anim Sci 36: 145–148
Nakanaga K, Yamanouchi K, Fujiwara K (1986) Protective effect of monoclonal antibodies on lethal mouse hepatitis virus infections in mice. J. Virol 59: 168–171
Rawls WE, Tompkins WAF (1975) Destruction of virus-infected cells by antibody and complement. In: Notkins AL (ed) Viral immunology and immuno-pathology. Academic Press, New York, pp 99–111
Taguchi F, Makino S, Fujiwara K (1982) Antigenic differentiation of mouse hepatitis viruses by neutralization test. Microbiol Immunol 26: 741–745
Talbot PJ, Salmi AA, Knobler RL, Buchmeier MJ (1984) Topographical mapping of epitopes on the glycoproteins of murine hepatitis virus 4 (JHM): correlation with biological activities. Virology 132: 250–260
Talbot P, Buchmeier MJ (1985) Antigenic variation among murine coronaviruses: evidence for polymorphism on the peplomer glycoprotein E2. Virus Res 2: 317–328
Towbin H, Stachelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Wege H, Stephenson JR, Koga M, Wege H, ter Meulen V (1981) Genetic variation of neurotropic and non-neurotropic murine coronaviruses. J Gen Virol 54: 67–74
Wege H, Siddel ST, ter Meulen V (1982) The biology and pathogenesis of coronaviruses. Curr Top Microbiol Immunol 99: 165–200
Wege H, Dorries R, Wege H (1984) Hybridoma antibodies to the murine coronavirus JHM: characterization of epitopes on the peplomer protein (E2). J Gen Virol 65: 1931–1942
Yewdell JW, Frank E, Gerhard W (1981) Expression of influenza A virus internal antigens on the surface of infected P815 cells. J Immunol 126: 1814–1819
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Lecomte, J., Cainelli-Gebara, V., Mercier, G. et al. Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Archives of Virology 97, 123–130 (1987). https://doi.org/10.1007/BF01310740
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DOI: https://doi.org/10.1007/BF01310740
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