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⇱ Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody | Archives of Virology | Springer Nature Link


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Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody

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Summary

Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immuno-fluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.

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Author information

Author notes
  1. V. Cainelli-Gebara

    Present address: Laboratoria Especial de Immunologia Viral, Instituto Butantan, S~ao Paulo, Brazil

Authors and Affiliations

  1. Centre de recherche en virologie, Institut Armand-Frappier, Ville de Laval, Québec, Canada

    J. Lecomte, P. J. Talbot & G. Lussier

  2. Centre de recherche en immunologie, Institut Armand-Frappier, Ville de Laval, Québec, Canada

    V. Cainelli-Gebara, G. Mercier, S. Mansour & D. Oth

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Lecomte, J., Cainelli-Gebara, V., Mercier, G. et al. Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody. Archives of Virology 97, 123–130 (1987). https://doi.org/10.1007/BF01310740

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  • DOI: https://doi.org/10.1007/BF01310740

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