VOOZH about

URL: https://pubmed.ncbi.nlm.nih.gov/32156330/

⇱ Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020 - PubMed

Clipboard, Search History, and several other advanced features are temporarily unavailable.
Skip to main page content
👁 Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

👁 Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Abstract

The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.

Keywords: COVID-19; Corona Virus; Infectious disease; Laboratory Diagnostics; Molecular Testing; Real-Time PCR; SARS-CoV-2; outbreaks.

PubMed Disclaimer

Conflict of interest statement

None declared.

Figures

👁 Figure 1
Figure 1
Cumulative numbers of suspected COVID-19 samples tested during week 5 and 6 2020, Bavaria (n = 675)
👁 Figure 2
Figure 2
Example image of real-time RT-PCR curves of the E gene assay with unspecific signals at late cycles, Bavaria, February 2020
👁 Figure 3
Figure 3
Flowchart and routine timeline of the SARS-CoV-2 diagnostic workflow at the LGL, Bavaria, February 2020

References

    1. Rothe C, Schunk M, Sothmann P, Bretzel G, Froeschl G, Wallrauch C, et al. Transmission of 2019-nCoV infection from an asymptomatic contact in Germany. N Engl J Med. 2020;NEJMc2001468.; Epub ahead of print. 10.1056/NEJMc2001468 - DOI - PMC - PubMed
    1. World Health Organization (WHO). Disease outbreak news: novel coronavirus—Thailand (ex-China). Geneva; WHO;14 Jan 2020. Available from; https://www.who.int/csr/don/14-january-2020-novel-coronavirus-thailand-e...
    1. World Health Organization (WHO). Disease outbreak news: novel coronavirus—Japan (ex-China). Geneva; WHO;17 Jan 2020. Available from; https://www.who.int/csr/don/17-january-2020-novel-coronavirus-japan-ex-c...
    1. World Health Organization (WHO). Disease outbreak news: novel coronavirus—Republic of Korea (ex-China). Geneva; WHO; 21 Jan 2020. Available from; https://www.who.int/csr/don/21-january-2020-novel-coronavirus-republic-o...
    1. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DKW, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):2000045. 10.2807/1560-7917.ES.2020.25.3.2000045 - DOI - PMC - PubMed

MeSH terms

Substances

LinkOut - more resources

Full text links
👁 Ingenta plc full text link
Ingenta plc Free PMC article
Cite
Send To

NCBI Literature Resources

MeSH PMC Bookshelf Disclaimer

The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Unauthorized use of these marks is strictly prohibited.