2013 - Volume 2, Issue 5 (September - October), Sep 2013
"ANTIFUNGAL ACTIVITY OF SELECTED MEDICINAL PLANT EXTRACTS AGAINST PLANT PATHOGENIC FUNGI; Rhizoct... more "ANTIFUNGAL ACTIVITY OF SELECTED MEDICINAL PLANT EXTRACTS AGAINST PLANT PATHOGENIC FUNGI; Rhizoctonia solani, Colletotrichum
musea and Fusarium oxysporum
MLMC Dissanayake* and JAN Jayasinghe
Department of Export Agriculture, Faculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanaka, Belihuoya, Sri Lanka
ABSTRACT
The aim of this work was to find an alternative to chemical fungicides currently used in the control plant pathogenic fungi Rhizoctonia solani ,Colletotrichum musae and Fusarium oxysporum,. The antifungalactivity of the methanol extracts of six medicinal plants used in native medicine in Sri Lanka is reported.Allplant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution (MID)against above fungi. The media amended with methanol and recommended fungicide for respective fungalstrain were consider as negative and positive control respectively.Results showed that radial growth in all thethree tested organisms was significantly impaired (p<0.05) by the addition of the extracts in the culturemedium used. The test fungi differed in their reaction to the different extracts but on the whole, growth inhibition increased with the concentration of each extract. The most active extracts, shows a marked effect of the 20% methanol extracts from sweet flag with inhibition values of 91%, 86% and 84 % for F. oxysporum,R.solani and C.mucea whereas those from wild basil inhibited the growth of the same pathogens by 89%, 84% and 74%.The results showed minimal inhibitory concentrations (MIC) were 5 % (v/v) for sweet flag and wild basil and 20% (v/v) for all other plant crude extracts. Out of six plants extract screened, wild basil and sweet flag showed more than 80% fungal inhibition after 6 hour immersion and other extracts could not exceed 60% inhibition after any exposure time. The study revealed that methanol crude extract of sweet flag and wild basil exhibit strong fungistatic and fungicidal activities against tested fungi. These results support the potential use of these plant extracts in the management of diseases caused by tested plant pathogenic fungi.
Keywords: Antifungal activity, Fusarium oxysporum,Rhizoctonia solani and Colletotrichum musae, plant
extracts, sweet flag and wild basil" 
Uploads
Papers by Asanga Jayasinghe
musea and Fusarium oxysporum
MLMC Dissanayake* and JAN Jayasinghe
Department of Export Agriculture, Faculty of Agricultural Sciences, Sabaragamuwa University of Sri Lanaka, Belihuoya, Sri Lanka
ABSTRACT
The aim of this work was to find an alternative to chemical fungicides currently used in the control plant pathogenic fungi Rhizoctonia solani ,Colletotrichum musae and Fusarium oxysporum,. The antifungalactivity of the methanol extracts of six medicinal plants used in native medicine in Sri Lanka is reported.Allplant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution (MID)against above fungi. The media amended with methanol and recommended fungicide for respective fungalstrain were consider as negative and positive control respectively.Results showed that radial growth in all thethree tested organisms was significantly impaired (p<0.05) by the addition of the extracts in the culturemedium used. The test fungi differed in their reaction to the different extracts but on the whole, growth inhibition increased with the concentration of each extract. The most active extracts, shows a marked effect of the 20% methanol extracts from sweet flag with inhibition values of 91%, 86% and 84 % for F. oxysporum,R.solani and C.mucea whereas those from wild basil inhibited the growth of the same pathogens by 89%, 84% and 74%.The results showed minimal inhibitory concentrations (MIC) were 5 % (v/v) for sweet flag and wild basil and 20% (v/v) for all other plant crude extracts. Out of six plants extract screened, wild basil and sweet flag showed more than 80% fungal inhibition after 6 hour immersion and other extracts could not exceed 60% inhibition after any exposure time. The study revealed that methanol crude extract of sweet flag and wild basil exhibit strong fungistatic and fungicidal activities against tested fungi. These results support the potential use of these plant extracts in the management of diseases caused by tested plant pathogenic fungi.
Keywords: Antifungal activity, Fusarium oxysporum,Rhizoctonia solani and Colletotrichum musae, plant
extracts, sweet flag and wild basil"