Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate‐based cholera toxin inhibitors

“…A 10 μM solution of LecA was injected in all cases and μ eff shifts were optimized. The μ eff were experimentally obtained using equation 1 : where L t is the total capillary length, L d is the capillary length to the detector, V is the applied voltage, t P the migration time of the protein ( Aizpurua-Olaizola et al. 2018 ).…”

“…The obtained values were plotted vs. the carbohydrate concentration. Plots were fitted using the software Prism 8.0 (GraphPad Software Inc., La Jolla, CA, USA) applying nonlinear regression and assuming a 1:1 binding stoichiometry according to equation 2 : where Δμ eff is the effective mobility difference of LecA obtained at a specific carbohydrate concentration compared to the one obtained when no carbohydrate was added to the BGE, B max is the maximum mobility shift, C r is the carbohydrate concentration, and K d is the dissociation constant ( Aizpurua-Olaizola et al. 2018 ).…”

“…Affinity chromatography is a process of separation a biomaterials of a mix, depends on a very specified macromolecular bind interaction among the bio materials and other matter. The specified kind of bind interaction based on the bio materials of benefit, antigen and antibody, enzyme and substrate, receptor and ligand, or proteins plus nucleic acid (18). Bind reactions were considerably used for separation different biomaterials.…”

“…Affinity chromatography is utilize with many test, involved nucleic acids purifying, proteins purifying (24) of cell extract, also purifying of blood. the use of affinity chromatography, first for isolate protein which bound a assured piece of proteins which not bound which specified piece (25).…”

“…[16,17] One of possible approaches is the modern paper-based sensor of Lateral flow assay (LFA), [18,19] which can be utilized for qualitative, semiquantitative, and in some scenario quantitative analysis and has capabilities such as portability, rapid assay time, cost effectiveness, low limit of detection, high sensitivity/selectivity, low quantity of sample volume required, no washing steps are necessary, robustness, and user-friendly method. [20][21][22][23][24] In addition, they have stability over wide spectrum of external conditions, high potential for commercialization, ease of device fabrication, and advantage over primary technology for their easy shipment to any countries. Regarding the fast development of LFAs as the most used POC sensor, Dong et al provided a systematic review of SARS-CoV-2 detection technologies, especially, LFA-based POC technique, as an insightful guidance and direction for developing tools towards the fast and large-scale diagnosis and analysis of SARS-CoV-2 to control public healthcare and support effective long-term pandemic management.…”