Transcriptomics articles from across Nature Portfolio

Massive analyses of RNA transcripts from rodents, monkeys and humans reveal hallmarks of ageing that could expedite the development of anti-ageing interventions.

Spatial maps of gene expression in whole human embryos across several weeks of early development offer insights into the molecular programs that drive organogenesis.

Two new methods show that bulk-trained sequence models can be efficiently adapted to predict gene regulation and variant effects at single-cell resolution.

Isoforms are seldom considered in genetic variant interpretation. Here the authors map common and rare variants to alternative isoforms, which can be highly expressed and tissue-specific. A common DPP9 variant maps to a lung expressed alternative isoform, with the missense variant altering activity.

Blood-based RNA and DNA methylation profiles reveal chronic coronary disease subtypes with distinct risk of cardiovascular events, improving cardiovascular risk assessment with validation in independent cohorts.

SL‑Smart‑seq3xpress is a highly sensitive single-cell RNA sequencing method that is compatible with organisms that undergo trans‑splicing and enables high‑resolution analysis of transcriptional and genomic heterogeneity in single cells.

Tintinnidorin proteins form a resistant material that self-assembles into the shells of tintinnid ciliates, which are unicellular planktonic eukaryotes. The material’s properties make it promising for biomaterial design.

Genetic analysis of the Dri1–Rhs1 complex sheds light on the link between the thermosensitivity of protein translation and the upper temperature limit for cell growth in fission yeast.

The authors optimize and apply in vivo AAV-Perturb-seq to systematically profile GWAS-linked major depressive disorder risk genes and their corresponding transcriptomic features in the mouse brain.

Spatial omics is moving from atlas-building to clinical translation, with the potential to reshape diagnosis, prognosis and therapy selection. Realizing this promise now requires scalable assays, standardized workflows, rigorous validation and regulatory-ready integration to convert spatial biology into practical tools for precision medicine.

Massive analyses of RNA transcripts from rodents, monkeys and humans reveal hallmarks of ageing that could expedite the development of anti-ageing interventions.

Spatial maps of gene expression in whole human embryos across several weeks of early development offer insights into the molecular programs that drive organogenesis.

Cellular-ageing clocks based on gene activity could be more sensitive than previous measures.

Two new methods show that bulk-trained sequence models can be efficiently adapted to predict gene regulation and variant effects at single-cell resolution.

In mice, motherhood induces transcriptional changes in the brain that endure beyond short-term hormonal shifts. Postpartum stress disrupts these patterns.